Libraries

SeSaM-Biotech offers SeSaM gene libraries for customers with in-house R&D to enable access of these customers to the innovative SeSaM technology.

SeSaM (Sequence Saturation Mutagenesis) is a patented method to create diversity on the gene level higher than the diversity generated by conventional methods such as error-prone PCR. The fact is that random mutagenesis methods produce only one nucleotide exchange per codon which leads to 39.2% of possible mutations. If two nucleotide exchanges per codon are achieved, this diversity increases to above 90%. This is what SeSaM is based on.

Why is SeSaM the ideal mutagenesis method?

• SeSaM targets every single protein position
• SeSaM exchanges an amino acid by all the other 19 amino acids with a probability above 0.9
• SeSaM has a built-in recombination option
• SeSaM libraries have reduced hotspots that leads to decrease in screening needs