Protein Engineering

Directed Evolution and Rational Design


Enzymes are nature’s way of catalyzing chemical reactions. Directed evolution is a collection of protocols through which natural enzymes are evolved in the laboratory towards non-natural properties. In the last 2 decades, using directed evolution, industry built up on nature’s success to develop clean ways to get to the products needed in modern world. Since the 1989 Exxon Valdez oil spill in Alaska was solved by bacteria degrading oil, mankind has turned to biotechnological methods such as directed evolution to answer many worldwide problems such as pollution due to waste coming from the industry, fossil fuel shortage, diseases or even poverty.


Since nature did not evolve enzymes to be the perfect biocatalyst and tolerate industrial conditions, we use protein engineering to enhance the enzymes’ performances under industrial conditions. Such enzyme evolution is performed by introducing mutations on the gene level in order to obtain altered enzyme variants with improved properties. We are able to effectively optimize enzymes towards:


  • higher activity/yield
  • enhanced thermo- or pH-stability
  • increased organic solvent resistance
  • improved specificity
  • larger or narrowed substrate spectrum
  • protease resistance
  • decreased subsrate or product inhibition
  • reduce sequence similarity with patented enzymes


Protein engineering as part of the QuESt-service


SeSaM-Biotech provides its Quality Enzyme Solutions for the use in industrial processes for fine chemicals, pharmaceuticals, cosmetics, food and nutrients and beverages, feedstocks, textiles, pulp and paper as well as biofuels.



Learn more about QuESt here or get in touch with us.