Assay Development

“A specific assay with high through-put and simple readout is the key to receive improved enzyme variants in a reasonable time-frame”



SeSaM-Biotech integrates biological, physical and chemical expertise in the development of highly specific assays to screen enzyme mutant libraries.

Depending on the desired through-put, the assays are designed on the following platforms:


through-put platform readout
low GC. HPLC chromatogram (peaks)
medium TLC, tube arrays colorimetric
high microtiter plate (96, 384 well) colorimetric, fluorometric
very high agar plate (semi-quantitative) colorimetric, zone of clearance
ultra-high FACS (semi-quantitative) fluorometric


Assay development includes test trials plus optimization of assay parameters and conditions.

Feel free to get in touch with us to learn about available assay systems and how we can support your R&D.

Assay development as part of the QuESt-service


SeSaM-Biotech has a wide variety of ready-to-use assay formats and systems available for most enzymes which are employed in the QuESt-service.

We are proud to say that 100 % of our Directed Enzyme Evolution projects achieved significantly improved variants, when the enzyme was actively expressed and the assay validated for Directed Evolution.


The following Enzymes have been evolved or have assays readily available:

  • Proteases
  • Lipases
  • Phytases
  • Amylases
  • Esterases
  • Peroxidases
  • Cyclases
  • Cellulases
  • P450 Monooxygenases
  • Sugar Isomerases
  • Laccases
  • Glucose Oxidases
  • Pectinases
  • Alcohol Dehydrogenases
  • Reductases
  • Polymerases
  • Lyases
  • Kinases
  • Methyltransferases
  • Deiminases
  • Hydantoinases
  • Epoxygenases

Learn more about QuESt here or get in touch to set up your Directed Evolution project.