Protein Engineering & Directed Evolution

Protein Engineering – specifically Directed Evolution – is the way to unlock the vast potential of nature’s enzymes and thus bring benefits to products, people and the planet.

While enzymes are the foundation of basically all reactions in living organisms on the molecular level, nature did not evolve enzymes to be perfect biocatalysts that tolerate conditions present in industrial products and processes.

Protein engineering, more specifically directed enzyme evolution, allows to adapt enzymes to these “un-natural” environments by introducing mutations on the gene level that result in improved properties and features of the enzyme.


We are able to effectively optimize enzymes towards:


  • Higher activity/yield
  • Enhanced stability at high/low pH
  • Enhanced stability at high/low temperatures
  • Enhanced long-term stability
  • Increased organic solvent resistance
  • Improved specificity
  • Larger or narrowed substrate spectrum
  • Protease resistance
  • Decreased subsrate or product inhibition
  • Reduce sequence similarity with patented enzymes



Want to learn more about our Directed Evolution expertise?

Protein engineering as part of the QuESt-service


Protein Engineering is a central part of SeSaM-Biotech’s QuESt-service, our all-in-one package for Directed Enzyme Evolution. We evolve enzymes for every industry ranging from fine chemicals, pharmaceuticals, molecular biology, diagnostics and cosmetics over food, nutrition and beverages, feedstocks to textiles, pulp and paper as well as biofuels.




The following enzymes have been evolved or have assays readily available:

  • Proteases
  • Lipases
  • Phytases
  • Amylases
  • Esterases
  • Peroxidases
  • Cyclases
  • Cellulases
  • P450 Monooxygenases
  • Sugar Isomerases
  • Laccases
  • Glucose Oxidases
  • Pectinases
  • Alcohol Dehydrogenases
  • Reductases
  • Polymerases
  • Lyases
  • Kinases
  • Methyltransferases
  • Deiminases
  • Hydantoinases
  • Epoxygenases

Learn more about QuESt here or get in touch with us..